The Women's Road Race event at the 2010 South American Games was held at 9:00 on March 22.
| Gold | Silver | Bronze |
| Ana Paola Villegas Colombia |
Rosane Kirch Brazil |
María Luisa Calle Colombia |
Race distance: 73.2km
| Rank | Rider | Time | Time Behind | Speed |
|---|---|---|---|---|
| Ana Paola Villegas (COL) | 2:10:57 | 33.539 | ||
| Rosane Kirch (BRA) | 2:14:03 | +3:06 | ||
| María Luisa Calle (COL) | 2:15:09 | +4:12 | ||
| 4 | Serika Ortiz (COL) | 2:16:50 | +5:53 | |
| 5 | Marcia Silva (BRA) | 2:23:40 | +12:43 | |
| 6 | Leidy Natalia Ruiz (COL) | s.t. | ||
| 7 | Paula Andrea Grandon (CHI) | 2:25:53 | +14:56 | |
| 8 | Uenia Souza (BRA) | s.t. | ||
| 9 | Lorena Maria Villamil (COL) | s.t. | ||
| 10 | Maria Eugenia Constante (ECU) | s.t. | ||
| 11 | Myrsa Analia Gaona (PAR) | 2:28:56 | +17:59 | |
| 12 | Flor Palma dos Santos (CHI) | 2:29:02 | +18:05 | |
| 13 | Barbara Cecilia Iturbe (CHI) | s.t. | ||
| 14 | Consuelo Gwynne Rodríguez (CHI) | s.t. | ||
| 15 | Claudia Aravena Cortes (CHI) | s.t. | ||
| 16 | Valeria Escobar (BOL) | 2:35:22 | +24:25 | |
| 17 | Tania Castro (ARG) | 2:36:13 | +25:26 | |
| Talia Ayelen Aguirre (ARG) | DNF | LAP | ||
| Caterine Priviley (ARG) | DNF | LAP | ||
| Valeria Pintos (ARG) | DNF | LAP | ||
| Olga Elena Muñoz (CHI) | DNF | |||
| Janildes Silva (BRA) | DNF | |||
| Valeria Teresita Muller (ARG) | DNF | |||
| Daiana Almada (ARG) | DNF | |||
| Daniela Mishell Carvajal (ECU) | DNS |